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Literature summary extracted from

  • Li, X.; Ma, J.; Zhang, Q.; Zhou, J.; Yin, X.; Zhai, C.; You, X.; Yu, L.; Guo, F.; Zhao, L.; Li, Z.; Zeng, Y.; Cen, S.
    Functional analysis of the two cytidine deaminase domains in APOBEC3G (2011), Virology, 414, 130-136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.4.B9 incorporation of hA3G CD1 and CD2 domain mutants into HIV-1. Cotransfection of plasmids coding HIV-1 vif and plasmids containing either wild-type or mutant hA3G in 293T cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.5.4.B9 additional information CD2-2, possessing the deamination activity, incorporated efficiently into HIV-1 is unable to mutate viral cDNA. Construction of three A3G mutants CD1-1, CD2-2 and CD2-1, which contain duplicate CD1 domain, duplicate CD2 domain, and position switched CD domain, respectively. The two CD domains are functionally equivalent in virion encapsidation and the interaction with HIV-1 Vif of hA3G, whereas CD domain switch or replacement greatly affect the sensitivity to Vif-induced degradation, editing and antiviral activity of hA3G. The switch of the CD domain affects nucleotide sequence preference pattern of the deaminase. The mutants show a nucleotide sequence preference pattern Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.B9 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.B9 additional information the preferred sequence context for wild-type hA3G is GG. In the CD2-1 mutant variants, both TGG and GGG are preferred, while the wild-type prefers TGG Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.B9 More A3G contains two cytidine deaminase domains. The CD2 domain possesses the deamination activity Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.5.4.B9 A3G
-
 Homo sapiens
3.5.4.B9 APOBEC3G
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.5.4.5 evolution APOBEC3G belongs to an APOBEC superfamily containing at least 11 members Homo sapiens
3.5.4.5 malfunction CD domain switch or replacement greatly affect the sensitivity to Vif induced degradation, editing and antiviral activity of hA3G, but the replacement or switch of CDs has no effect on hA3G viral incorporation, overview. The mutants show a nucleotide sequence preference pattern Homo sapiens
3.5.4.5 additional information HIV-1 Vif protein interacts with hA3G as a part of a Vif-Cul5-SCF complex, resulting in polyubiquitination and proteosomal degradation of hA3G. The hA3G/Vif interaction is a prerequisite for the degradation of hA3G Homo sapiens
3.5.4.5 physiological function the two cytidine deaminase domains of the cytidine deaminase act as an anti-HIV-1 host factor. The two CD domains of A3G are functionally equivalent in virion encapsidation and the interaction with HIV-1 Vif off A3G, overview Homo sapiens